This page has moved to here: http://www.protocol-place.com/cell-culture/coating-6-well-or-12-well-plates-with-collagen/
This protocol was updated on 3/20/13. In the previous version, collagen was dried down to a film overnight while the solution still contained ions from PBS/DPBS. Upon drying, salt crystals were destroying the collagen. The new protocol calls for (1) allowing the collagen to polymerize and then (2) gently washing the collagen with distilled water before drying overnight in order to prevent crystal formation.
Also, the order in which the reagents are combined was altered so that the collagen gets added last. This way, any potential effects from drastically changing the collagen’s pH is avoided by adding the collagen to a buffered solution (pH is buffered by the PBS).
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